Nanomechanical Probes of Single Corneal Epithelial Cells: Shear Stress and Elastic Modulus
نویسندگان
چکیده
Living human corneal epithelial cells have been probed in vitro via atomic force microscopy, revealing the frictional characteristics of single cells. Under cell media, measured shear stresses of 0.40 kPa demonstrate the high lubricity of epithelial cell surfaces in contact with a microsphere probe. The mechanical properties of individual epithelial cells have been further probed through nanometer scale indentation measurements. A simple elastic foundation model, based on experimentally verifiable parameters, is used to fit the indentation data, producing an effective elastic modulus of 16.5 kPa and highlighting the highly compliant nature of the cell surface. The elastic foundation model is found to more accurately fit the experimental data, to avoid unverifiable assumptions, and to produce a modulus significantly higher than that of the widely used Hertz–Sneddon model.
منابع مشابه
Indentation quantification for in-liquid nanomechanical measurement of soft material using an atomic force microscope: rate-dependent elastic modulus of live cells.
In this paper, a control-based approach to replace the conventional method to achieve accurate indentation quantification is proposed for nanomechanical measurement of live cells using atomic force microscope. Accurate indentation quantification is central to probe-based nanomechanical property measurement. The conventional method for in-liquid nanomechanical measurement of live cells, however,...
متن کاملEffect of Defects on Mechanical Properties of Graphene under Shear Loading Using Molecular Dynamic Simulation
Graphene sheet including single vacancy, double vacancy and Stone-Wales with armchair and zigzag structure was simulated using molecular dynamics simulation. The effect of defects on shear’s modulus, shear strength and fracture strain was investigated. Results showed that these shear properties reduce when the degrees of all kinds of defects increase. The dangling bond in SV and DV defected gr...
متن کاملModulus of elastic pdf
The modulus of elasticity Youngs modulus E is a material property, that. From the Hooks law the modulus of elasticity is defined as the ratio of the stress to.modulus of elasticity of the cultured SC cells examined in this study, in central, non-nuclear regions, ranged between. Johnson B.all require an effective E, the Youngs modulus or elastic modulus, to quantify. Elastic moduli, such as the ...
متن کاملEx vivo expansion of limbal stem cells is affected by substrate properties.
Limbal epithelial stem cells play a key role in the maintenance and regulation of the corneal surface. Damage or destruction of these cells results in vascularisation and corneal opacity. Subsequent limbal stem cell transplantation requires an ex vivo expansion step and preserving cells in an undifferentiated state remains vital. In this report we seek to control the phenotype of limbal epithel...
متن کاملStudy of Stone-wales Defect on Elastic Properties of Single-layer Graphene Sheets by an Atomistic based Finite Element Model
In this paper, an atomistic based finite element model is developed to investigate the influence of topological defects on mechanical properties of graphene. The general in-plane stiffness matrix of the hexagonal network structure of graphene is found. Effective elastic modulus of a carbon ring is determined from the equivalence of molecular potential energy related to stretch and angular defor...
متن کامل